Laparoscopic hemicolectomy has become the standard of care for the surgical management of various colonic pathologies. Proper instrument setup and systematic counting protocols are essential components of surgical safety and procedural efficiency. The setup protocol for laparoscopic hemicolectomy shown in this video is based on established guidelines for sterile technique and encompasses instrument arrangement, the initial count, and discussion of fluid management on the sterile field. The setup includes organized placement of laparoscopic instruments, trocars, sponges, sharps, and essential equipment. This setup promotes surgical safety, reduces procedural delays, and minimizes the risk of retained surgical items.