The success of all surgical procedures depends, in part, on adherence to sterile technique and well-ordered arrangement of instruments and other items to support optimal efficiency. This educational video demonstrates a complete back table and Mayo stand setup for an open cholecystectomy, including preparation of the sterile field and organization of instruments and supplies in order to prepare for the initial surgical count with a circulator. Educational materials, such as this video, that provide guidance on proper setup methods, can benefit surgical technology students and new practitioners by encouraging them to create consistent perioperative practices.