This video demonstrates lumpectomy and sentinel lymph node biopsy using the Lumicell intraoperative imaging system in a patient with breast cancer. The case illustrates step-by-step surgical technique, from preoperative dye administration to final skin closure, with a focus on fluorescence-guided margin assessment. The Lumicell system enabled real-time detection of residual tumor cells, guiding additional resection as needed. Key procedural details, including margin orientation, hemostasis, and probe handling, are shown. This resource highlights the potential of intraoperative fluorescence imaging to improve surgical outcomes and reduce reoperation rates in breast-conserving therapy.