This article describes the technique of performing an end-to-end arterial anastomosis on a 1mm diameter rat femoral artery. Microsurgery anastomosis is a technique required for free flap transfers, transplant surgery and other surgical applications. This video article shows the microsurgical anastomosis technique in detail, covering aspects that are difficult to grasp without direct visualization. The laboratory environment is ideal tor practicing the delicate and meticulous maneuvers of microsurgery and for becoming familiar with the microscope and specialized tools involved. We hope this article will familiarize the prospective trainee prior to taking courses at our laboratory.